OSfinder: A Tool for Accurate Orthology Mapping

Draw Synteny Maps

Step 0: Make Sure Requirements.
Our Perl script for drawing dot-plots requires the GNUplot package (4.2 or later version) to be installed.

Step 1: Draw Dot-Plots.
A dot-plots visualizes the genomic positions of the orthologous segments identified between two chromosomes. To draw dot-plots between the X chromosome of Homo sapiens and the X chromosome of Mus musculus, type as below. Here, we assume that the "chrom_map" file for Homo sapiens defines the chromosome ID of the human X chromosome as "80", and that the "chrom_map" file for Mus musculus defines the X chromosome ID of the mouse X chromosome as "135".

% cd osfinder_v*_*/
% ./scripts/draw_dot_plot.pl -i osfinder_results/hsa-mmu.blastp.os.txt -a 80 -b 135

Then, a PNG file named "hsa-mmu.blastp.os.80vs135.png" will be created in the "osfinder_results" directory. By opening the PNG file, you can display the dot-plot between the human X chromosome and the mouse X chromosome as below.


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